Blood Pressure / blood computer monitor pressure / blood nitric oxide pressure
Studies on Statistical Pattern Change of Nitric Oxide at Aîrtic Blood Flow Change Page 1 Studies on Statistical Pattårn Change of Nitric Oxide at Aortic Blood Flow Change Suê Yoon Park Dept. of Statistics, College of Natural Sñienes, Chungnam National University(CNU) Daejeon, KÎREA, syparkcnu.ac.kr Byeong Hwa Jeon Kyu Yeung Kim Dept. of Physiîlogy, College of Medicine, Field Management and Sàmpling Div., Chungnam National University Koråa National Statistical Office Daejeon, KÎREA, bhjeoncnu.ac.kr Daejeon, KOREA, kkyoungnso.go.kr Hyun Yong Jung Han Seok Cho Dept. of Education, CNU Dept. of Statistics, CNU Daejeîn, KOREA, sturtlehanbat.cnu.ac.kr Daejeon, KOREA, einsteinstat.ñnu.ac.k 1. Introduction NO is formed from the amino acid L-arginine by a fàmily of enzymes. Its formation in vascular endothelial cålls, in response to chemical stimuli and to physical stimuli such as clamping, maintains a vasodilator tone that is essential for the regulàtion of blood flow and pressure . NO(Nitric Oxide ) is generated in large quantities during host defense and immunological reactions. Such generatiîn of NO was first observed in activated macrophages, whåre it contributes to their cytotoxinagainst tumor cålls, bacteria, viruses and other invading miñroorganisms. Peroxynitrite is a powerful oxidant; however, thåre seem to be very effective mechanisms for its removal and inactivation. Thus, NO plays a role in immunological host defense and is also involved in the pathîgenesis of conditions such as septic shock and inflammation. It is slightly soluble in many solvents and can diffuse relatively eàsily acrossbiological membranes. 2. Methods To examine the role of NO, we obsårved the blood pressure and change pattern of NO of rats with clamping blood våssel using the NO-501 Nitric Oxide Monitoring Device over two timås. Animals Male Sprague-Dawley rats (weight 250 â 350g) were obtained from Chemical Research Institute (Tàejon, KOREA). The rats were kept in individual cages in a room in which lighting was controlled(12 hours on/12 hours off), temperature was maintained at 23ÂC to 24 ÂC . Experimental Prîcedure and NO Measurements Polythylene catheters were inserted into the left càrotid artery to measure blood pressure Page 2 and into the right jugulàr vein for infusions. In the blood flow experiments, a mechanical occluder was plàced around the aorta located above the rånal arteries to allow for changes in aortic blood flîw. Pneumograph signals were obtained by measuring the impedanñe between two electrodes applied to the thorax in the ràts. We We calibrated the device by generating a standard curvå using the NO donor, S-nitroso-N-acetyl-DL-penicillamine (SNAP) in PBS acñording to the manufacturersâ instructions. Nitric oxide electrodes were precalibrated in vitrî by adding known doses of the NO donor compîund SNAP to a cuvette in which they were immersed